Inosine utilizes both A2aR and A3R to inhibit LPS-induced TNFα in vivo. Mice were injected intraperitoneally with 100 mg/kg inosine followed by 10 mg/kg LPS and the level of TNFα in the serum collected 1 hour after LPS injection was assessed. This figure shows that inosine inhibited LPS-induced TNFα production in wild-type (A,C,E), A2aR^-/- (B), and A3R^-/- (D) mice, but did not inhibit TNFα in A2aA3R^-/- (F) mice, which lacked both receptors. Representative results from 1 of 3 separate experiments.