Pf EMP1 is associated with coated vesicles and Maurer clefts. Trophozoite stage IRBCs were treated with AlF, processed for immunoelectron microscopy and probed with an α-Pf EMP1 antibody. Pf EMP1 associated with (A) the parasite compartment and coated vesicles in the RBC cytosol and (B) Maurer clefts. Scale bar = 70 nm.