Two-color flow cytometric characterization and intracellular cytokine expression in primed T-cell populations. Representative experiment of 3 independent experiments (each using a different donor). Shown are the results of FACS analysis, giving CD8 and CD56 prevalence within the primed population resulting from lysate- or AAV/HM1.24/Neo-loading techniques. In addition, the intracellular prevalences of IFN-γ and IL-4 within primed and stimulated mixed T-cell populations are shown, resulting from lysate- and AAV/HM1.24/Neo-loading techniques. Note that the use of AAV/HM1.24/Neo loading resulted in a higher IFN-γ/IL-4 ratio and a lower CD56/CD8 ratio.