Dosage-dependent CTL killing. Shown are CTLs that resulted from AAV loading-dose responses of 3 donors (each experiment performed in quadruplicate) at the top and a compilation of the results from all 3 donors at the bottom. These experiments were similar to those discussed in Figure 5 except that the DCs were initially loaded (infected at day 0) with different amounts of the AAV/HM1.24/Neo virus, as indicated. “Lysate” refers to 293T cell lysates. This cell lysate contained no virus and was thus a mock infection. Target cells were generated by introduction of the HM1.24 gene into autologous PBLs 4 days before the CTL assay, as described in “Materials and methods.” Note that the resultant CTLs effected a level of killing that directly correlated with the amount of virus originally used for loading DCs at day 0.