Target specificity (autologous targets). AAV/HM1.24 generated a CTL response against (A) HM1.24-positive (▪) and –negative (□) autologous targets. (A) Shown are CTL responses of 3 donors (each experiment performed in quadruplicate) and (B) a compilation of the results from all 3 donors. These data demonstrate that the cytotoxic response resulting from AAV/HM1.24/Neo vector loading of DC is antigen specific in a fully autologous system. CTLs were generated by AAV/HM1.24/Neo-loading (1 mL virus or approximately 2 × 109 eg) DCs and T cells stimulated as described in Figure 2. Two target types were used: autologous PBMCs (no HM1.24 antigen) and PBMCs that had been preloaded with M1.24 antigen 4 days before the chromium release assay. Note that only the HM1.24-positive PBMC targets were killed by the CTLs.
