HM1.24 mRNA expression in infected DCs. Total RNA was isolated from 2 cell populations: mock-infected and AAV/HM1.24/Neo-infected adherent monocytes at 72 hours after infection. These samples were analyzed by RT-PCR and PCR, as indicated, for the presence of HM1.24 RNA, as described in “Materials and methods.” The positive control was the PCR product resulting from using the AAV/HM1.24/Neo vector plasmid as a template. Another control was RT-PCR analysis for the cellular TFIIB mRNA. Note that only RNA from cells infected with AAV/HM1.24/Neo virus resulted in an appropriate RT-PCR–sized product, whereas mock-infected cells did not.