AAV/HM1.24/Neo vector, producer cell lines, and experimental scheme. (A) A structural map of the AAV/HM1.24/Neo virus (also known as dl6-95/HM1.24p5/NeoSV40) shows the names of the components at the top. TR (black boxes) refers to the AAV terminal repeats. P5 (bent arrow) refers to the AAV p5 promoter. SV40 (bent arrow) refers to the SV40 early enhancer/promoter. Boxes labeled HM1.24 and Neo represent the indicated open-reading frames. (B) Titer analysis of the AAV/HM1.24/Neo stock used in these experiments. (C) Depiction of the experimental protocol.