Figure 1.
![Figure 1. Specificity of TFCD and P-TFCD antibodies in dot blot assays. TF cytoplasmic domain peptides (nonphosphorylated, double-phosphorylated at Ser253 and Ser258 [P-Ser253, 258] or single-phosphorylated peptides (P-Ser253 or P-Ser258) with or without pretreatment with potato acid phosphatase [PAP]) were spotted as 1-μL dots at 3 different concentrations on a nitrocellulose membrane that was hybridized with 1:1000 diluted antiserum to the unphosphorylated (αTFCD, left) or the phosphorylated (αP-TFCD, right) TF cytoplasmic domain.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/12/10.1182_blood-2003-04-1149/6/h82335302001.jpeg?Expires=1722788500&Signature=vVhDk4xYIQBUW79-ALAvcEEPm0oRMpZkyqR3vtPzQ43cgAhU2IF0ZA67jG6DMI~bojX284W2yOTXM~R0~gSPeMvinBYpHZHrR1s53whF7LstDWIy2tsFc-IguzHaWiia974qkCLLFhvu79ju-radUpJQefuWTZbnMvgsjtYdFYQYpqhz-NX21sc~L0dr-vm9~BHtUdlTMP1A1ajyfwKOdQbdGLSksaFROQtFbbI1hkCbvnVHitYwdEus~N2nTd~dbtBf-o9eZTAr9ZTl0dvTMUcyAkw34~iSDvfnFobb3Ju~kLTYSoiJTdOg0QYayFQjetX~58b1ARxH9aO3kmRVAw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Specificity of TFCD and P-TFCD antibodies in dot blot assays. TF cytoplasmic domain peptides (nonphosphorylated, double-phosphorylated at Ser253 and Ser258 [P-Ser253, 258] or single-phosphorylated peptides (P-Ser253 or P-Ser258) with or without pretreatment with potato acid phosphatase [PAP]) were spotted as 1-μL dots at 3 different concentrations on a nitrocellulose membrane that was hybridized with 1:1000 diluted antiserum to the unphosphorylated (αTFCD, left) or the phosphorylated (αP-TFCD, right) TF cytoplasmic domain.
