Figure 3.
Figure 3. Allele-specific RNA PCR. (A) The RNA PCR product from both adult α-globin genes of most non-AKR strains is bisected by an MspI restriction site. (B) Allele-specific RNA PCR on blood from HS26+(nonAKR)/+(AKR) non-AKR × AKR F1 mice. Controls are water (W) and C57BL6 (B6). (C) Allele-specific RNA PCR on bone marrow from HS26floxed(nonAKR)/+(AKR), HS26+(nonAKR)/+(AKR), and HS26ΔNFE2(nonAKR)/+(AKR) non-AKR × AKR F1 mice.

Allele-specific RNA PCR. (A) The RNA PCR product from both adult α-globin genes of most non-AKR strains is bisected by an MspI restriction site. (B) Allele-specific RNA PCR on blood from HS26+(nonAKR)/+(AKR) non-AKR × AKR F1 mice. Controls are water (W) and C57BL6 (B6). (C) Allele-specific RNA PCR on bone marrow from HS26floxed(nonAKR)/+(AKR), HS26+(nonAKR)/+(AKR), and HS26ΔNFE2(nonAKR)/+(AKR) non-AKR × AKR F1 mice.

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