American Society of Hematology

Figure 1.

$Effect of lovastatin, FTI-277, and GGTI-298 on farnesylation and geranylgeranylation. (A) RPMI 8226 cells were treated for 2 days with solvent control or lovastatin (30 μM) alone or in the presence of mevalonate (meva; 100 μM), GGOH (10 μM), or FOH (10 μM). For the dose response, RPMI 8226 cells were treated with solvent control or lovastatin (1, 2, 3, 4, 5, 10, 30, 50, or 100 μM). After protein isolation, prenylation status of DnaJ and Rap1a was determined by Western blot analysis. The faster-migrating band represents prenylated protein, and the slower band represents unprenylated protein. Data are representative of at least 3 independent experiments. (B) RPMI 8226 cells were treated for 2 days with solvent control, FTI-277 (2.5, 5, 10, 15, 20, or 30 μM), or GGTI-298 (2.5, 5, 10, 15, 20, or 30 μM). After protein isolation, prenylation status of DnaJ and Rap1a was determined by Western blot analysis. Data are representative of at least 3 independent experiments. (C) RPMI 8226 cells were exposed to solvent control or lovastatin (30 μM) alone or in the presence of mevalonate (100 μM), GGOH (10 μM), or FOH (10 μM). After a 2-day incubation, protein was separated in membrane and cytosolic fractions. RhoA was identified by Western blot analysis. Data are representative of 3 independent experiments.$

Effect of lovastatin, FTI-277, and GGTI-298 on farnesylation and geranylgeranylation. (A) RPMI 8226 cells were treated for 2 days with solvent control or lovastatin (30 μM) alone or in the presence of mevalonate (meva; 100 μM), GGOH (10 μM), or FOH (10 μM). For the dose response, RPMI 8226 cells were treated with solvent control or lovastatin (1, 2, 3, 4, 5, 10, 30, 50, or 100 μM). After protein isolation, prenylation status of DnaJ and Rap1a was determined by Western blot analysis. The faster-migrating band represents prenylated protein, and the slower band represents unprenylated protein. Data are representative of at least 3 independent experiments. (B) RPMI 8226 cells were treated for 2 days with solvent control, FTI-277 (2.5, 5, 10, 15, 20, or 30 μM), or GGTI-298 (2.5, 5, 10, 15, 20, or 30 μM). After protein isolation, prenylation status of DnaJ and Rap1a was determined by Western blot analysis. Data are representative of at least 3 independent experiments. (C) RPMI 8226 cells were exposed to solvent control or lovastatin (30 μM) alone or in the presence of mevalonate (100 μM), GGOH (10 μM), or FOH (10 μM). After a 2-day incubation, protein was separated in membrane and cytosolic fractions. RhoA was identified by Western blot analysis. Data are representative of 3 independent experiments.

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