Antileukemic agents that generate intracellular ROS induce the nuclear accumulation of BACH2. BACH2-overexpressing RAJI cells (no. 67) were treated with drugs at the indicated concentrations—control, not treated; MTX, 0.02 μg/mL; VCR, 0.01 μg/mL; VP16, 2 μg/mL; DNR, 0.2 μg/mL; and Ara-C, 0.1 μg/mL—a day after culture. Cells were fixed and then stained for Bach2 proteins and for DNA. The merged images show BACH2 (green) and DNA (blue). Original magnification, × 400.