Figure 4.
Measurement of intracellular ROS production using a redox-sensitive dye and FACS. BACH2-overexpressing (no. 75) and control (pDL2) RAJI clones were seeded (2 × 105/mL) into 12-well plates and treated with 0.02 μg/mL MTX (A), 0.01 μg/mL VCR (B), 2 μg/mL VP16 (C), 0.2 μg/mL DNR (D), or 0.1 μg/mL Ara-C (E) for 24 hours. After treatment, the cells were stained with DCFH-DA and analyzed by flow cytometry as described in “Materials and methods.” Control cells are indicated by open area, and treated cells by filled area. The results are representative of 3 separate experiments.
