Figure 2.
Figure 2. Single-cell RT-PCR and S1 nuclease protection assay. Qualitative analysis of single-cell RT-PCR assays. (A) Huβ/huδ (266 bp) as well as βmaj/βmin (343 bp) transcripts were amplified and distinguished after EcoRI digestion.31 wt indicates wild-type mouse total bone marrow RNA; ln2, ln2 total bone marrow RNA; and -ctl, negative control. (B) Lanes 1-6 are representative examples of single-cell RT-PCR performed on ln2 c-Kit+/CD31high/Ly-6C- cells.(C) Lanes 1-9 are representative examples of single-cell RT-PCR performed on human CD34+ cells.

Single-cell RT-PCR and S1 nuclease protection assay. Qualitative analysis of single-cell RT-PCR assays. (A) Huβ/huδ (266 bp) as well as βmaj/βmin (343 bp) transcripts were amplified and distinguished after EcoRI digestion.31  wt indicates wild-type mouse total bone marrow RNA; ln2, ln2 total bone marrow RNA; and -ctl, negative control. (B) Lanes 1-6 are representative examples of single-cell RT-PCR performed on ln2 c-Kit+/CD31high/Ly-6C- cells.(C) Lanes 1-9 are representative examples of single-cell RT-PCR performed on human CD34+ cells.

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