American Society of Hematology

Figure 5.

$Figure 5. Selective association of cpERM with the insoluble cytoskeleton in resting PBT cells. PBT cells were either untreated or treated with SDF-1α (100 ng/mL, 1 minute), calyculin A (CA; 25 nM, 5 minutes), or staurosporine (ST; 500 nM, 5 minutes). Data shown are Western blots of cell fractions detecting carboxy-threonine phosphorylated ERM (mAb 297s), total ERM (polyclonal anti-ezrin), and β-actin (mAb AC-15) on duplicate membranes. Note that the soluble fraction is diluted 5-fold relative to the insoluble fraction.$

Selective association of cpERM with the insoluble cytoskeleton in resting PBT cells. PBT cells were either untreated or treated with SDF-1α (100 ng/mL, 1 minute), calyculin A (CA; 25 nM, 5 minutes), or staurosporine (ST; 500 nM, 5 minutes). Data shown are Western blots of cell fractions detecting carboxy-threonine phosphorylated ERM (mAb 297s), total ERM (polyclonal anti-ezrin), and β-actin (mAb AC-15) on duplicate membranes. Note that the soluble fraction is diluted 5-fold relative to the insoluble fraction.

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