Flow cytometric analysis demonstrates rapidity of cpERM dephosphorylation. PBT cells were stimulated in suspension with SDF-1α (100 ng/mL) for the indicated time and fixed, and cpERM proteins were labeled using mAb 297s and FITC-conjugated donkey antirat IgG. The fluorescence intensity of 10 000 cells/sample was measured by flow cytometry. Data were gated for single, intact cells and plotted as events versus log fluorescence intensity.