SDF-1α and SLC induce dephosphorylation of moesin. Lysates compared are either from untreated cells (0 min) or cells treated for the indicated time with the chemokines SDF-1α or SLC (100 ng/mL). Data shown are Western blots of whole cell lysates using mAb 297s (A; cpERM) which specifically recognizes moesin phosphorylated at T558 (as well as phospho-ezrin), compared to total ERM protein (B) detected with polyclonal antiezrin on a duplicate membrane. (A) Time course of dephosphorylation induced by chemokines; (B) inhibition of dephosphorylation by pertussis toxin (PTX). Samples on the right were preincubated for 2 hours with 100 ng/mL PTX. For comparison, Western blot was also performed with antibody specific for phosphorylated MAPK (B; pMAPK).