Southern blot analysis showing clonal repopulation of mice receiving transplants with limiting numbers of Sca1⁺lin^– BCR-ABL–transduced (GFP⁺)+/+ and SHIP^–/– cells. Genomic DNA was digested with EcoR1 and probed for GFP. EcoR1 cleaves once within the retroviral sequence; therefore, each fragment represents a unique site of retroviral integration. Lanes 1-10 and 11-18 show the results for DNA isolated from the spleen or liver of sick mice injected with 10³ to 2 × 10⁴ Sca-1⁺lin^–GFP⁺ BCR-ABL–transduced cells from +/+ cells and SHIP^–/– mice, respectively. Molecular size markers are also indicated. M indicates marker.