Allogeneic ALAK cells have minimal effects on myeloid reconstitution after BMT. B6-Ly5.2 BMCs (5 × 10⁷) and ALAK cells (5 × 10⁷) from B10 or B10.D2 mice were cultured for 24 hours, as described in “Materials and methods.” (A) A fraction of cocultured cells was cultured in semisolid colony assay media in triplicate for another 7 days, as described in “Materials and methods.” Pooled data from 2 independent experiments are shown. (B) B6 mice were irradiated at 8.5 Gy and underwent transplantation with the cocultured cells at 3 × 10⁶ BMCs per mouse based on the cell numbers at the initiation of coculture, as described in “Materials and methods.” At various days after BMT, spleen and femurs were harvested (3 mice per group per time point), splenocytes (2.5 or 5 × 10⁵) and BMCs (5 × 10⁴) were cultured in semisolid colony assay media in triplicate. Colonies were enumerated on a stereoscope at day 7, as described in “Materials and methods.” Representative data and SEMs from 2 independent experiments are shown.