American Society of Hematology

Figure 2.

Schematic representations of cocultures, in vitro assays, and BMT. NK or ALAK cells with or without pretreatment with F(ab′)2 fragments of anti-Ly49 antibodies were cultured with C1498 or P815, BMCs contaminated with C1498, or BMCs alone for 24 hours before further use, as described in “Materials and methods.” (A) In vitro colony or proliferation assays. (B) Ex vivo purging protocol for BMCs contaminated with C1498 and BMT. (C) Adaptation of ex vivo purging protocol to examine the effects on BMCs.

Schematic representations of cocultures, in vitro assays, and BMT. NK or ALAK cells with or without pretreatment with F(ab′)₂ fragments of anti-Ly49 antibodies were cultured with C1498 or P815, BMCs contaminated with C1498, or BMCs alone for 24 hours before further use, as described in “Materials and methods.” (A) In vitro colony or proliferation assays. (B) Ex vivo purging protocol for BMCs contaminated with C1498 and BMT. (C) Adaptation of ex vivo purging protocol to examine the effects on BMCs.

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