![Figure 7. The expression of Bcl-XL and Bad phosphorylation contribute to survival function of FLT3/ITD in FLT3/ITD-32D cells. (A) In FLT3/ITD-32D cells, after 24-hour treatment with Bcl-XL/antisense (Bcl-XL/AS) or Bcl-XL/sense (Bcl-XL/S) oligonucleotides and an additional 4-hour treatment with or without kinase inhibitors (10 μM LY294002 [LY] and 10 μM PD98059 [PD]), each sample was lysed and immunoblotted by anti–Bcl-XL antibody. Each cell lysate was immunoprecipitated by anti-Bad antibody and immunoblotted by antiphospho Bad specific antibodies. Immunoblotting by antiactin antibody is for the control of total protein level. (B) Inhibitory effect of Bcl-XL/AS and kinase inhibitors on the viability of FLT3/ITD-32D cells. After 24-hour treatment with each oligonucleotide, LY (10 μM) and PD (10 μM) were or were not applied. The data shown are means and SDs of 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/8/10.1182_blood-2002-12-3813/6/h82035076007.jpeg?Expires=1724137083&Signature=lMFQAb8OJsXYGvfY-O1phZK7KxPF9cohkLAm~JmnUo~fAfDa3qvd27oIjsgsAinmovjdseOLl7wNWQ~26JaZzN3619KxMVhCPM84hbt2VNbJJZcU0q-k0EJjxLoQq3eC19aEHtEy~60iVzAH2XY5SG-B2QYD3tvViUSigYxchnwEcnsA3uUQZ8MJ5RV-Zj4v034Tew8m9~bCbT38xZB6UU1SzCLBhAGcfD4FlwWB6pj~-5Vsr5XPQ9S1Rb9uNE5rs9feu-yesH9j93PbvaEtn0IbBrWUj52FIKkwaVqzFC~vqX1zBiaKyhstH9MOsRzwrT9AeWnJF4dFKIN3LSX2~A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The expression of Bcl-XL and Bad phosphorylation contribute to survival function of FLT3/ITD in FLT3/ITD-32D cells. (A) In FLT3/ITD-32D cells, after 24-hour treatment with Bcl-XL/antisense (Bcl-XL/AS) or Bcl-XL/sense (Bcl-XL/S) oligonucleotides and an additional 4-hour treatment with or without kinase inhibitors (10 μM LY294002 [LY] and 10 μM PD98059 [PD]), each sample was lysed and immunoblotted by anti–Bcl-XL antibody. Each cell lysate was immunoprecipitated by anti-Bad antibody and immunoblotted by antiphospho Bad specific antibodies. Immunoblotting by antiactin antibody is for the control of total protein level. (B) Inhibitory effect of Bcl-XL/AS and kinase inhibitors on the viability of FLT3/ITD-32D cells. After 24-hour treatment with each oligonucleotide, LY (10 μM) and PD (10 μM) were or were not applied. The data shown are means and SDs of 3 independent experiments.
