Figure 6.
Figure 6. Inhibition of FLT3/ITD kinase activity down-regulates the expression of Bcl-XL and Bad phosphorylation in FLT3/ITD-32D cells. (A) In treatment with AG1296 at increasing doses for 4 hours, each cell lysate was immunoprecipitated by anti-Bad antibody and immunoblotted by antiphospho Bad specific antibodies. (B) In parental 32D cells stimulated with IL-3 and FLT3/ITD-32D cells, each cell lysate at the indicated times was immunoblotted by anti–Bcl-2, Bcl-XL, Bax and Bak, and Mcl-1 antibodies. (C) The mRNA level of Bcl-XL was examined by reverse-transcription PCR assay using 30 amplification cycles. For the control of RNA recovery, β-actin expression was used.

Inhibition of FLT3/ITD kinase activity down-regulates the expression of Bcl-XL and Bad phosphorylation in FLT3/ITD-32D cells. (A) In treatment with AG1296 at increasing doses for 4 hours, each cell lysate was immunoprecipitated by anti-Bad antibody and immunoblotted by antiphospho Bad specific antibodies. (B) In parental 32D cells stimulated with IL-3 and FLT3/ITD-32D cells, each cell lysate at the indicated times was immunoblotted by anti–Bcl-2, Bcl-XL, Bax and Bak, and Mcl-1 antibodies. (C) The mRNA level of Bcl-XL was examined by reverse-transcription PCR assay using 30 amplification cycles. For the control of RNA recovery, β-actin expression was used.

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