Figure 7. The rate of kallikrein cleavage of CHO cell–expressed HK light chain. (A) Western blot detects a specific 40-kDa band of the recombinant (His)6–HK4-6 in all 3 strains and shows the disappearance of the (His)6 tag band as it is cleaved by kallikrein. (B) Quantitative densitometric analysis of the (His)6–HK4-6. The rate of cleavage as a function of time was analyzed. ○ indicates Lewis; □, Fischer; and ▿, Buffalo. Mean ± SEM, n = 3. The Lewis recombinant (His)6-tagged HK cleavage was significantly faster than observed in the Fischer or Buffalo rat recombinants.
Figure 7.

The rate of kallikrein cleavage of CHO cell–expressed HK light chain. (A) Western blot detects a specific 40-kDa band of the recombinant (His)6–HK4-6 in all 3 strains and shows the disappearance of the (His)6 tag band as it is cleaved by kallikrein. (B) Quantitative densitometric analysis of the (His)6–HK4-6. The rate of cleavage as a function of time was analyzed. ○ indicates Lewis; □, Fischer; and ▿, Buffalo. Mean ± SEM, n = 3. The Lewis recombinant (His)6-tagged HK cleavage was significantly faster than observed in the Fischer or Buffalo rat recombinants.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal