Restriction enzyme analysis of 492-bp PCR products from Lewis and Fischer rats. The 492-bp PCR product was subjected to analysis using ScaI restriction enzyme. The products were analyzed using an Agilent NA500 Labchip as described in “Materials and methods.” The first lane indicates Lewis PCR product without ScaI restriction enzyme; the second lane, Lewis PCR product with ScaI; the third lane, Fischer PCR product without ScaI; and the fourth lane, Fischer PCR product with ScaI. The 369-bp and 112-bp cleavage products are shown. The top band in all the lanes is a high–molecular weight internal marker.