Figure 3.
![Figure 3. 729HTLVRex- stable cell line contains the Rex mutation and does not produce detectable Rex. (A) HTLV-1 genome fragment containing the Rex start codon was amplified by PCR from genomic DNA of 729wtHTLV-1 and 729HTLVRex- cells. PCR-amplified product was incubated in the presence (+) or absence (-) of SphI, separated on 2% agarose gel, and visualized by ethidium bromide staining. (B) Rex-1 immunoprecipitation. 729, 729wtHTLV-1, and 729HTLVRex- cells were metabolically labeled with [35S] methionine-cysteine. Cell lysates were immunoprecipitated with polyclonal antibody against the HTLV-1 Rex in the presence of protein A-sepharose. The sizes (in daltons, indicated on the left) were determined by comparison to protein markers.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/12/10.1182_blood-2003-05-1490/6/h82335288003.jpeg?Expires=1724247142&Signature=tVIc10VffK~QPwGF6s3-lASbtViaSh-Ua2Ft2hyZRwNFgV4k-oqAV2ZLwSMMjkcAFOy~a2R62izR4BofTgK8VvahuCmhHw7SRESKoetpseeoB1JBPDHUmklqn2OGDgrPBqij6aTb7DhCmLqQPBz465Lo69BxoQG9le4zt5eDvYUxcRvQLHEiNydOQk8-psXL75rAyArtsxg0Q8YmC1WpdUgZny~baiLgPaIAccWZWGywc8ANCpPTFcDVIgxi6IGJQBKD8Ak5dayfRviEHZ7X5TWm~TmAytWfRZ-ep8saaYKGWcPzv4XxGNjjiZ3Ew1xiqIWDY60DrJ8yJTg8eSEoxg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
729HTLVRex- stable cell line contains the Rex mutation and does not produce detectable Rex. (A) HTLV-1 genome fragment containing the Rex start codon was amplified by PCR from genomic DNA of 729wtHTLV-1 and 729HTLVRex- cells. PCR-amplified product was incubated in the presence (+) or absence (-) of SphI, separated on 2% agarose gel, and visualized by ethidium bromide staining. (B) Rex-1 immunoprecipitation. 729, 729wtHTLV-1, and 729HTLVRex- cells were metabolically labeled with [35S] methionine-cysteine. Cell lysates were immunoprecipitated with polyclonal antibody against the HTLV-1 Rex in the presence of protein A-sepharose. The sizes (in daltons, indicated on the left) were determined by comparison to protein markers.
