Western blots from HUVECs cultured static or at 0.3 or 2.0 Pa. (A) IL-8 and P-selectin for HUVECs that had been cultured for 24 hours static or under flow, and then a further 4 hours with 100 U/mL TNF. (B) TNF receptors 1 and 2 (p55TNFR and p75TNFR) for HUVECs that had been cultured for 24 hours static or under flow. Actin is shown as a loading control in each case. Positions of bands for P-selectin and IL-8 were determined by comparisons to purified proteins run in separate lanes and to molecular weight standards. Bands for TNF receptors were consistent with known molecular weights. Western blots are from individual experiments, which were repeated on 2 to 4 occasions with similar results.