Evaluation of p53 protein in Fancc^-/- cells. Radiation-induced p53 protein in Fancc-deficient cells. MEFs were treated with 10 Gy IR and cultured for the indicated time periods, and protein extracts (50 μg) were analyzed by Western blotting using antibody specific to p53. A representative of 3 independent experiments is shown. Total protein levels are normalized using actin as a control, shown in the bottom panel.