Time course analysis of the production of ROS in monocytes and granulocytes. Graphs show production of ROS in monocytes (A) and granulocytes (B). Leukocytes were incubated with anti-D–opsonized RBCs (□) for the indicated times, and ROS production was analyzed as in Figure 1. Controls, including the stimulants E coli (•), fMLP (▪), and PMA (○), are shown. The data are expressed as fold change in ROS production ± SD and calculated by the formula: percentage of fluorescence (FL1)–stimulated cells/percentage of fluorescence (FL1)–nonstimulated cells.