Sustained ICN1 expression in CD34^hi thymic lymphoid progenitors results in a skewed production of γδ T cells at the expense of αβ T cells. (A) Flow cytometry analysis of CD3 versus TCRαβ or TCRγδ expression on electronically gated GFP⁺ progenies derived from ICN1-transduced (ICN1) or mock-transduced (GFP) CD34^hi thymic lymphoid progenitors at day 32 in FTOC. Numbers represent percentages in each quadrant. Percentages (B) and absolute numbers (D) of TCRγδ⁺ cells recovered from lobes seeded with ICN1- (▪) or GFP-transduced (○) progenitors at the indicated days of culture. (C) TCRαβ⁺/TCRγδ⁺ cell ratios recovered from mock-transduced (open bars) or ICN1-transduced (filled bars) thymic lymphoid precursors (14.3 ± 6.5 and 0.3 ± 0.2, respectively). Values are the mean ± SD of absolute cell number ratios observed at 11 different time points along FTOCs from 4 independent experiments.