N-terminal cleavage or Asp33Ala mutation appears to enhance homooligomerization of Bax in mitochondrial membranes. Inducible Bax expression was performed in the presence of 40 μM ALLM for 24 hours. The heavy membrane was collected, and cross-linking was performed as described in “Materials and methods.” Then, membranes were lysed and 40μg lysate was analyzed by SDS-PAGE and Western blots with anti-T7 tag antibody.