Figure 4.
![Figure 4. p18 Bax has a reduced half-life that can be enhanced by ALLM. (A) The half-life of WT and p18 Bax was first determined by [35S]methionine metabolic labeling and pulse-chase as described in “Materials and methods.” (B) The half-life of WT, Asp33Ala, p18, and Glu6Ala Bax was determined again using the cyclohexamide method as described in “Materials and methods.” (C) The effect of ALLM on Bax protein half-life was determined by cyclohexamide method. Half-life was determined by densitometry analysis.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/7/10.1182_blood-2003-01-0211/6/h81935030004.jpeg?Expires=1726840590&Signature=2upUnLlGIPRROyU6CCrUSDeLZFpi8Aa2KcW-Xs~nWMuA41SoloAInS2h4ax9fKE3sh3lli3DSE8dsOxjG65n3hYXHizGnxClksf62wOCaWzqaLC~uiASNGCJqlcTq2uYPk7M-MCoMNIF~hqexp9bSOeHGwoVSNo4Tnj9c1~5I~auoTI7cQFYfrALaD1RVwaWAzpXS6f-rXn0-gNRI4Uv8OrGas-eXvPsmv44lj7Gzo409hkhDiDGE8S3T6TSiYexUUewgajhcZ56~iAbwms5XliXgv0nh9M7gn2T9Jm7BmhKf-xaLMr8foe43BCDne48cNOoHuRRpKiE2CJX6ljFmA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
p18 Bax has a reduced half-life that can be enhanced by ALLM. (A) The half-life of WT and p18 Bax was first determined by [35S]methionine metabolic labeling and pulse-chase as described in “Materials and methods.” (B) The half-life of WT, Asp33Ala, p18, and Glu6Ala Bax was determined again using the cyclohexamide method as described in “Materials and methods.” (C) The effect of ALLM on Bax protein half-life was determined by cyclohexamide method. Half-life was determined by densitometry analysis.
