IL-5, CCL1, and CCL5 production by human MCs and human monocytes. Human MCs were precultured with or without IFN-γ and then exposed to LPS for 16 hours. Control cells were incubated similarly, except that LPS was omitted. Positive control cells were precultured with IgE and then activated with anti-IgE for 16 hours. Human monocytes (Mono) were exposed to LPS for 16 hours. The cell supernatants were used for cytokine ELISAs.