Figure 5.
![Figure 5. Functional properties of purified subsets after IL-15-stimulation. The purified subsets were labeled with CFSE and stimulated with IL-15 (10 ng/mL). After 7 days the cells were restimulated with PMA/ionomycine and brefeldin A and stained intracellular for indicated cytokines followed by FACS analysis (gamma-1 [g-1] used as control mAbs). (A) CD8+ T cells purified from UCB. (B) Peripheral naive CD8+ T cells. (C) Cytotoxic CD8+ T cells (CD27-CD45RAbright). (D) Noncytotoxic CD8+ T cells (CD27+CD45RAdull/neg). Data are one representative experiment of 4 performed.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/7/10.1182_blood-2003-01-0183/6/h81934985005.jpeg?Expires=1722767399&Signature=b6ukUU39o~LLj08btb-4gJX1OPfFfKcEeGUHlOuyloMGuySTU4N8JP7SmBIQXwviDxk-DUjHNgYG-fVb0k2Q2XyPGn1axGQjUxM3nob0mZFGfob5aR0GU~eO0~nASv94zDgljuZwpRjlS~VBIWvj74xRj6AC2Nq5grGziXsFVt2SloT0vezQplkhcpZlL4-EQPKUJohRQPbJMp4aelb1rSvo6MemAqK1RhE9eE7MNxY6hvwrSA5nPQQOPhSGNyx0Re3o9V8A1YBMGE6w5CaFdQlMlDcX4Jk~EGxPh0bpw1A358~sQAhEyeN7B9J8tJZFfaJZnhlBhimc9973fc4zZg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Functional properties of purified subsets after IL-15-stimulation. The purified subsets were labeled with CFSE and stimulated with IL-15 (10 ng/mL). After 7 days the cells were restimulated with PMA/ionomycine and brefeldin A and stained intracellular for indicated cytokines followed by FACS analysis (gamma-1 [g-1] used as control mAbs). (A) CD8+ T cells purified from UCB. (B) Peripheral naive CD8+ T cells. (C) Cytotoxic CD8+ T cells (CD27-CD45RAbright). (D) Noncytotoxic CD8+ T cells (CD27+CD45RAdull/neg). Data are one representative experiment of 4 performed.
