Induction of molecular remission in the presence of infiltration with CD8⁺ cytotoxic killer cells. (A) TCR-γ chain PCR DGGE of CTCL patient 1 demonstrating a Vγ 1-8 band before treatment (lane 2), positive control MyLa cell line (lanes 1 and 3). Disappearance of tumor-specific clone after vaccination in residual erythematous lesion (lane 4). Immunohistologic staining of regressive CTCL lesion demonstrates infiltration of CD8⁺ (B) and TIA⁺ (C) T cells at the dermo-epidermal junction. Sections were stained using the APAAP technique and counterstained with hematoxylin. Original magnifications, × 400 (B) and × 200 (C).