Figure 2.
![Figure 2. Induction of PBL proliferation and IFN-γ secretion after DC vaccination. PBLs from patients 1 (Ai,Bi), 5 (Aii,Bii), 8 (Aiii,Biii), 9 (Aiv,Biv), and 10 (Av,Bv) before (▪) and after (□) vaccination were cultured for 7 days in RPMI containing 10% human AB serum in the presence of 100 μg/mL tumor lysate or PBL lysate. No lysate was added to negative controls (-). (A) Cell proliferation was assessed by measuring [3H]thymidine incorporation using a scintillation counter. Data are shown as mean ± SD of triplicate wells. (B) Supernatants from 3 wells were pooled after 7 days of incubation, and IFN-γ secretion was measured by ELISA. *Significant difference (P < .05).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/7/10.1182_blood-2002-08-2455/6/h81935017002.jpeg?Expires=1724243057&Signature=wpmz0yZReCCe2W-EzxzLxv3vWJduqNSmuq~dwkW7mSdvOz4bz7OiYLLnJf5e~r91S3mlAmGso14StMeQeGDLUXSbNmzaRO42vRbBs~rf9GmID-0rtwtUe9DLyFZwhUwz8ubKS9wthUZfZ2aAb4mbHbZ-TetuKzU8dPMWXVUfpIMv4sjUHGnUhSlwp8BGsO221rsD0XLbip-i7y91HtQsGBy-FAoCH~M6-shxqio6Uwds8x3Pf8GAxeojs9DXNw~mz22mOXTFOziWh-cCyidIWdf2I2DMXkG5bRBI0OqsKwnc5C02yX7c-1TmCl1TKMQu-7sKz~4jLckfJsjS40XDKw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Induction of PBL proliferation and IFN-γ secretion after DC vaccination. PBLs from patients 1 (Ai,Bi), 5 (Aii,Bii), 8 (Aiii,Biii), 9 (Aiv,Biv), and 10 (Av,Bv) before (▪) and after (□) vaccination were cultured for 7 days in RPMI containing 10% human AB serum in the presence of 100 μg/mL tumor lysate or PBL lysate. No lysate was added to negative controls (-). (A) Cell proliferation was assessed by measuring [3H]thymidine incorporation using a scintillation counter. Data are shown as mean ± SD of triplicate wells. (B) Supernatants from 3 wells were pooled after 7 days of incubation, and IFN-γ secretion was measured by ELISA. *Significant difference (P < .05).
