The effect of ligation of MyD-1 on the inhibition of TNFα induced by other pathogen products. Human PBMCs were cultured for 24 hours with either 100 ng/mL LPS, 1 μg/mL Zymosan A, or 1000 U/mL PPD in the presence or absence of murine anti-human MyD-1 or the isotype control antibody, after which supernatants were analyzed by ELISA for TNFα. The results shown are the mean production of TNFα (means ± 1 SD, *P < .01, **P < .005 versus IgG1 control) for (A) LPS, (B) Zymosan A, or (C) PPD cultures. These data are representative of 3 experiments.