Immunolocalization of GST-RBD, GST-PBD, and GST-WBD in COS7 cells carrying RhoAQL, Rac1QL, and Cdc42QL. COS7 cells transfected with cDNA encoding RhoAQL (B,F,L,P), Rac1QL (C,G,M,Q), and Cdc42QL (D,H,N,R) were fixed, permeabilized, and incubated with GST-RBD (A-D), GST-PBD (E-H), or GST-WBD (I-N). Anti-GST Ab was used as negative control (O-R). Scale bar, 10 μm. There were 3 experiments performed with similar results.