Neutralization of endogenous CCL2 results in cytoskeleton rearrangements and cell size modification in MDMs. (A) Dual-fluorescence CLSM analysis was performed on fixed MDMs double stained for α-tubulin (green) and actin (red) at different time points of culture. Shown is 1 representative experiment of 3 performed. (B) Dual fluorescence CLSM analysis was performed on MDMs maintained in the presence of different concentrations of the Ag affinity-purified polyclonal Ab to CCL2 or control Ab for 72 hours, fixed and double stained for α-tubulin and actin. The bars, indicated in the lower right panels, correspond to 20 μm.