Figure 4.
p210 BCR/ABL regulates the nuclear assembly of XPB and PCNA in response to UVC. (A) BaF3 and BaF3-p210 lymphoid cells and (B) 32D and 32D-p210 myeloid cells were irradiated or not with 10 J/m2 UVC. Nuclear localization of PCNA (red) and XPB (green) was detected on the cytospins prepared at time 0 and 6 hours after irradiation. DNA was counterstained by DAPI. Yellow color indicates the possible colocalization sites on PCNA and XPB. Results represent 2 independent experiments. Original magnification, × 1000. Expression of PCNA and XPB in total cell lysates before and after UVC was detected by Western analysis.
