Figure 1.
![Figure 1. Dissociation of radiolabeled WT- and ΔK-scuPA from soluble uPAR. suPAR-coated wells were incubated with 125I-WT-scuPA (5 [▾], 10 [▴], and 20 nM [▪]) or 125I-ΔK-scuPA (5 [▿], 10 [▵], and 20 nM [□]) for 2 hours at 22°C. Wells were washed 4 times, and the released radioactive ligand was counted at the indicated times. The lines represent the best fit to the single exponential decay model as determined using GraphPad software.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/10/10.1182_blood-2003-03-0949/6/h82235250001.jpeg?Expires=1722434687&Signature=oYquE~y0IF8O9miJnJ1DoqZH~b~boacOxUtQ7EfVlg0s~5sjvqrIuYiEml~LFbxne0-8kso4QmmqNol9l1mpm8JJkIY~5KlsLm2oP1Y9uqq7EHxtvFUNRK-UKxGIA-2kV6lE3s7njgIyaaJcDBDxUtyuCUfL2zU6o0KT-1amsS2qH4QyjYVKaVzOO3LOr08rfjmQBl2h~gF-QjzZz6-qIiaFgJ2Obised0V-dvXxmhMkPxzvzUiMNBK~UiTIe2nmZWa421ktXqaVJflBBnwNAP84-fzEAcTIMvmU2lG65zDPphVBXUyK9to3PLoR4r2gPc4~oUcCuUr8VJSeUAhTXA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Dissociation of radiolabeled WT- and ΔK-scuPA from soluble uPAR. suPAR-coated wells were incubated with 125I-WT-scuPA (5 [▾], 10 [▴], and 20 nM [▪]) or 125I-ΔK-scuPA (5 [▿], 10 [▵], and 20 nM [□]) for 2 hours at 22°C. Wells were washed 4 times, and the released radioactive ligand was counted at the indicated times. The lines represent the best fit to the single exponential decay model as determined using GraphPad software.
