C3 MSCs inhibit an allogeneic proliferative response in a dose-dependent manner. Responding BALB/c splenocytes (105 cells) were incubated for 4 days with either 5 μg/mL ConA or mitomycin-treated DBA1 splenocytes (105) or various ratios of DCs, with or without C3 MSCs (105 cells or various ratios). (A) C3 MSCs inhibit the TCR-independent (ConA) and dependent (allogeneic) T-cell proliferative response. The proliferative response corresponding to the average cpm of triplicates of ConA-induced T-cell proliferation was assigned the value of 100% ± SD. (B) C3 MSCs induce a dose-dependent inhibition of allogeneic T-lymphocyte proliferation. Results are expressed as the percent of T-cell proliferation obtained in the absence of MSCs ± SD. (C) C3 MSCs repress T-cell proliferative activity induced by allogeneic professional antigen-presenting dendritic cells. The T-cell proliferation induced by allogeneic splenocytes was assigned the value of 100% ± SD.
