Figure 1.
![Figure 1. The stable isotope-based dynamic metabolic profile (SIDMAP) of mammalian cells is based on 13C glucose carbon tracer rearrangements in metabolic intermediates. Schematic of pathways to ribose formation (1), glycolysis (2), and TCA cycle (3) from [1,2-13C2]glucose in cultured fibroblasts. This tracer broadly labels intermediary metabolites in cultured TRMA (-/-) cells that indicate substrate flow defects in comparison with wild-type (WT) (+/+) cells in low-thiamine medium. Using SIDMAP, the rate of glycolysis, pentose cycle ribose synthesis, TCA cycle anaplerotic flux, direct glucose oxidation, and de novo fatty acid synthesis are measured in one tracer experiment.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/10/10.1182_blood-2003-05-1537/6/h82235251001.jpeg?Expires=1724237521&Signature=EO0G0LJemjuiX8WA312srqRtPcfzsM6dHFz15WODgeoOHkJU700kflyYF0o-DNoes51ca2ddVsg4XXF2fe4eZkixzAulo8hmDtyoQ5CFU0TWOugQIgRUIMY9wy~ZmsocOyB60Y6IZOSG6MF95NtqCEUqQKpQkSqdwEoBHwVe9EhOJzebHdlQWs7s0iBhQrEIUd2ufZgxlWQDmp-95B27y3gEN6Dcy0vHLpt5I2VvQRIGSmi4wbeD6~KzDqaqapC0i61~CdDu~s6OmfUTOFiPWD54A1~cnkMKXt336WFeJzV3RB6qykkklYIrTz9S~OxLu8PtURgs~jSoTISjymRzRQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The stable isotope-based dynamic metabolic profile (SIDMAP) of mammalian cells is based on13C glucose carbon tracer rearrangements in metabolic intermediates. Schematic of pathways to ribose formation (1), glycolysis (2), and TCA cycle (3) from [1,2-13C2]glucose in cultured fibroblasts. This tracer broadly labels intermediary metabolites in cultured TRMA (-/-) cells that indicate substrate flow defects in comparison with wild-type (WT) (+/+) cells in low-thiamine medium. Using SIDMAP, the rate of glycolysis, pentose cycle ribose synthesis, TCA cycle anaplerotic flux, direct glucose oxidation, and de novo fatty acid synthesis are measured in one tracer experiment.
