Figure 2.
Figure 2. Polymerization. Polymerization of normal (thick lines) and BβArg14His (thin lines) fibrinogens by (A) thrombin, (B) batroxobin, and (C) desA fibrin monomers. Fibrinogen (0.2 mg/mL) was polymerized with thrombin or batroxobin (0.1 U/mL) in 20 mM HEPES, pH 7.4, 148 mM NaCl, 1 mM CaCl2. DesA fibrin monomers in 0.125% acetic acid were diluted 1:10 to a final concentration of 0.076 mg/mL in 100 μL of 40 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM CaCl2.

Polymerization. Polymerization of normal (thick lines) and BβArg14His (thin lines) fibrinogens by (A) thrombin, (B) batroxobin, and (C) desA fibrin monomers. Fibrinogen (0.2 mg/mL) was polymerized with thrombin or batroxobin (0.1 U/mL) in 20 mM HEPES, pH 7.4, 148 mM NaCl, 1 mM CaCl2. DesA fibrin monomers in 0.125% acetic acid were diluted 1:10 to a final concentration of 0.076 mg/mL in 100 μL of 40 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM CaCl2.

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