Figure 1.
![Figure 1. SDS-PAGE and fibrinopeptide release curves of normal and BβArg14His fibrinogens. (A) Thrombin-catalyzed release of FpA (squares) and FpB (circles) from normal [▪, •] and BβArg14His (□, •) fibrinogens. (B) Batroxobin-catalyzed release of FpA from normal (▪) and BβArg14His (•) fibrinogens. Conditions: 0.1 mg/mL fibrinogen, 0.1 U/mL thrombin or 0.005 U/mL Batroxobin in 20 mM HEPES, pH 7.4, 150 mM NaCl. (inset) 10% reducing SDS-PAGE gel of normal (lane 1) and BβArg14His (lane 2) fibrinogens, with molecular weight markers indicated on left.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/7/10.1182_blood-2003-01-0204/6/h81935034001.jpeg?Expires=1722709717&Signature=yV6MaTv76sVgVX9lwrJvxipihbfIpNyZkMavisYOcx~YYrVuyaxS3MPxx0Ihwk6sn4Lbe0FsjAapMacGqtKG1WAEJBKlxmMdCF~ezbuZ58bwGnahuGlp7YihozbUwYiTK17R3XqCBBJqXex04~rG-fPsg99cFypQZqhmyKHsUb2tg5amR0QeRzkHzbP5h3FWE2KxQA3XCDm3QvtoFBcM~BRsSzbpfX~cZrZzmKEKLcfUdfD8n5YNbaiDEeicyeAZr6zYLb-OiHS-i0rmgaXVU85~ZpIKXi0h8blBUciJgqLtEYO95AMjEuz5NLCEl4nyuY9ET8qzWP8k5Ua2VHfLdQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SDS-PAGE and fibrinopeptide release curves of normal and BβArg14His fibrinogens. (A) Thrombin-catalyzed release of FpA (squares) and FpB (circles) from normal [▪, •] and BβArg14His (□, •) fibrinogens. (B) Batroxobin-catalyzed release of FpA from normal (▪) and BβArg14His (•) fibrinogens. Conditions: 0.1 mg/mL fibrinogen, 0.1 U/mL thrombin or 0.005 U/mL Batroxobin in 20 mM HEPES, pH 7.4, 150 mM NaCl. (inset) 10% reducing SDS-PAGE gel of normal (lane 1) and BβArg14His (lane 2) fibrinogens, with molecular weight markers indicated on left.
