Cytokine profile of Jagged-1 primed naive T cells. (A) Cytokine profiles of unseparated (CD4 and CD8) naive T cells were analyzed in day-3 culture supernatants by CBA assay and ELISA for Th1/Th2 cytokines and TGF-β content, respectively. Results from 3 experiments are shown with a P value for the effect of Jagged-1 of P = .0135 for IL-2, P = .049 for IFN-γ, P = .57 for IL-10, P = .0144 for IL-5, and P = .0071 for TGF-β. (B) Intracellular staining for IL-4 and IFN-γ on day 7 of the unseparated cultures, and with gating on CD4⁺ or CD8⁺ T-cell populations.