Phenotypic characteristics of tolerized T cells. After a 7-day MLC in the absence or presence of Jagged-1, the responder cells were harvested, allowed to rest for 2 days, and analyzed by flow cytometry. (A) The blastlike (ie, large-sized) T cells emerged with distinct forward scatter (FSC) characteristics in the Jagged-1 MLC. (B) Annexin V staining showed a comparable level of apoptotic cells between control and Jagged-1 MLC (1 representative experiment of 3).