CIB associates with FAK in human platelets. (A) Immunofluorescence analysis of CIB and FAK localization in platelets throughout the stages of platelet adhesion to immobilized Fg. Scale bar, 1 μm. (B) Immunoprecipitation of CIB from platelet lysates of cells adhered to Fg or BSA. Western blot analysis with anti-FAK shows the association between CIB and FAK increases on platelet adhesion to Fg. (C) Immunoprecipitation of CIB from TRAP-activated platelet lysates in the presence or absence of RGDS. Western blot analysis with anti-FAK shows the association between CIB and FAK is blocked on inhibition of the ligand binding.