CIB induces focal adhesion formation. (A) Immunofluorescence images of mock- and CIB-overexpressed CHO cells adhered to fibronectin and stained for vinculin. Cells were observed for focal adhesion formation at 30, 60, and 120 minutes. (B) Quantitation of the number of focal adhesions formed per cell in both mock- and CIB-transfected cells at each time point as observed by vinculin staining. CIB overexpression significantly (P < .05) increases focal adhesion formation. (C) Immunofluorescent images as in panel A stained for phosphotyrosine (p-Tyrosine). (D) Quantitation of the number of focal adhesions formed in both mock- and CIB-transfected cells at each time point as observed by phosphotyrosine staining, in that CIB overexpression significantly (P < .05) increased the number and rate of development of focal adhesions. Scale bar (A,C), 10 μm. Error bars (B, D) indicate mean ± SEM of at least 3 independent experiments.