Figure 5.
![Figure 5. The effect of anti-Bcl-2-scFv-TAT on the mitochondrial membrane potential. RBL cells were exposed to 30 μg/mL anti-Bcl-2-scFv-TAT or nonspecific scFv-TAT for various periods of time and were then stained with MitoTracker Red, as described in “Materials and methods.” The changes in mitochondrial membrane potential were monitored by FACS. Control represents unstained cells. One representative experiment of 3 is shown. (A) Changes in fluorescence in RBL cells after 1 hour of treatment (left panel) and after 12 hours (right panel). (B) Mean fluorescence intensity monitored as function of the cell time treatment (anti-Bcl-2-scFv-TAT [□]; and nonspecific scFv-TAT [▦]).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/7/10.1182_blood-2002-12-3921/6/h81935002005.jpeg?Expires=1726826471&Signature=CSEN6KiLCt8xCAFRZ77zeFHoxeiZF81SNozDyPu2sqWWruxgYN-7P4NwIXvojmkXOBUKcDvPTxyP5KyqoNcZeszm22kW5Cu-5iAwcGyV4Lbabf8EttKLkL0ZDv48K3seewsDCsifb0qXViq~V~est3MwJt8ktN8nELr-OzTZ0wWbe6~F7XLoA51~kfmUYqgOF6aHp10GPv1WWlJ9WE5s1Fi96BO9hgFUeP6FGinBOi04fm4NpcGYjDhD8cSO9TZH-hFbs1YTKVaz8Fu6BSatPdVM2z4x2i9e-10xbOwvx-pmSWMOvFvUsCDLpo-~srzAsxIPdGm9G3Elm-XqHU7Z0g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The effect of anti-Bcl-2-scFv-TAT on the mitochondrial membrane potential. RBL cells were exposed to 30 μg/mL anti-Bcl-2-scFv-TAT or nonspecific scFv-TAT for various periods of time and were then stained with MitoTracker Red, as described in “Materials and methods.” The changes in mitochondrial membrane potential were monitored by FACS. Control represents unstained cells. One representative experiment of 3 is shown. (A) Changes in fluorescence in RBL cells after 1 hour of treatment (left panel) and after 12 hours (right panel). (B) Mean fluorescence intensity monitored as function of the cell time treatment (anti-Bcl-2-scFv-TAT [□]; and nonspecific scFv-TAT [▦]).
