Figure 2.
Figure 2. Translocation of scFv-TAT through the cell membrane. RBL cells (untreated, panel A) were exposed for 90 minutes to anti-Bcl-2-scFv-TAT at different concentrations (5 μg/mL, panel C; 15 μg/mL, panel D), and to anti-Bcl-2-scFv (10 μg/mL, panel B). The translocation of anti-Bcl-2-scFv-TAT into the cell cytoplasm was visualized using protein A-FITC, seen in the figure as white dots (C-D). Cells were observed under a fluorescent microscope at ×40 magnification.

Translocation of scFv-TAT through the cell membrane. RBL cells (untreated, panel A) were exposed for 90 minutes to anti-Bcl-2-scFv-TAT at different concentrations (5 μg/mL, panel C; 15 μg/mL, panel D), and to anti-Bcl-2-scFv (10 μg/mL, panel B). The translocation of anti-Bcl-2-scFv-TAT into the cell cytoplasm was visualized using protein A-FITC, seen in the figure as white dots (C-D). Cells were observed under a fluorescent microscope at ×40 magnification.

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