Effect of adoptively transferredAspergillus-pulsed DCs on the induction of antifungal T_Himmunity. Splenic DCs were pulsed and administered into mice subsequently infected intravenously with A fumigatus or C albicans as described in Figure 3. Antifungal T_H immunity was assessed in terms of cytokine production (ELISA) by antigen-stimulated CD4⁺ T splenocytes from mice with aspergillosis (A) or candidiasis (B) or cytokine gene expression by real-time PCR (C). ELISA and real-time PCR were done 6 days after the infection. ^*P < .05 (mice receiving pulsed DCs versus mice receiving unpulsed DCs). ▪, unpulsed DCs; ▨, conidia-pulsed DCs; ▦, conidial RNA-transfected DCs. Error bars indicate SE.